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The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.
Hong Kong's Hang Seng and the Shanghai Composite in mainland China were also down. The Kospi in South Korea, which was shut for a public holiday on Monday, fell by more than 7%.，更多细节参见safew官方下载
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