Agar was first introduced into the laboratory in 1881. Since then, microbiologists have depended on agar to create strong jellies. When microorganisms are streaked or plated onto this jellied surface and incubated, individual cells multiply into distinct colonies that scientists can easily observe, select, and propagate for further experiments. Many of the most important findings in biological research of the last 150 years or so — including the discovery of the CRISPR/Cas9 gene-editing tool — have been enabled by agar.4 Agarose, a derivative of agar, is also essential in molecular biology techniques like gel electrophoresis, where its porous gel matrix separates DNA fragments by size, enabling researchers to analyze and isolate specific genetic sequences.
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The old barges, once used to transport building materials, coal and food from the Port of London, have spent more than 30 years moored in Essex.
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This pattern has caused connection pool exhaustion in Node.js applications using undici (the fetch() implementation built into Node.js), and similar issues have appeared in other runtimes. The stream holds a reference to the underlying connection, and without explicit consumption or cancellation, the connection may linger until garbage collection — which may not happen soon enough under load.